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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Glutathione S-transferase (GST) is a group of enzymes that involved in the detoxification of xenobiotics. GST catalyzes the formation of the thiol group of glutathione to electrophilic xenobitocs. It utilizes glutathione to scavenge toxic substances that produced by oxidative stress. The GST Fluorometric Activity Assay Kit provides a simple, fluorescence-based in vitro assay for detecting the GST activity using fluorescence plate reader. The assay utilizes monochlorobimane (MCB), a dye that is nonfluorescent on its own, but emits blue (ex/em = 380/461 nm) when reacts with glutathione. The MCB-glutathione reaction is catalyzed by GST and the fluorescence levels are proportionally to the amounts of GST in the reaction. Therefore, the GST level can be simply detected by a fluorometer or a 96-well fluorometric plate reader.