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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Pyruvate is an important intermediate in several metabolic pathways. Pyruvate is produced from glucose via glycolysis. Pyruvate can be converted to fatty acids via acetyl CoA or to carbohydrates via gluconeogenesis. Pyruvate is also used to stimulate metabolism which then leads to loss of body weight. High levels of pyruvate are associated with genetic disorders and liver disease.
The Pyruvate Colorimetric/Fluorometric Assay Kit provides a simple and convenient way for detection of pyruvate in various biological samples such as culture and fermentation media, blood and cells based on colorimetric and fluorometric method. In the assay, pyruvate is oxidized by pyruvate oxidase via enzyme reactions to yield fluorescence (at Ex/Em = 535/587 nm) and color (at λ = 570 nm). The fluorescence or color intensity is proportional to pyruvate content. The assay provides a direct and automation-ready procedure for accurately measuring pyruvate concentration. The kit can detect 1 µM to 10 mM pyruvate.