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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Caspases (Cysteine-dependent aspartate-directed proteases) are a family of cysteine proteases that play important roles in apoptosis, inflammation and necrosis. Sequential activation of caspases plays an important role in cell apoptosis. Caspase-12 is an inflammatory caspase that is involved in processing and activating inflammatory cytokines such as interleukin 1 and interleukin 18. Inhibition of caspase-12 leads to a stronger inflammatory reaction to bacterial pathogens.
Caspase-12 Fluorometric Assay Kit provides a highly sensitive, simple and convenient way for detecting the ATAD-dependent caspase activity based on detection of cleavage of substrate ATAD-AFC. ATAD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin) emits blue light (λmax = 400 nm); while cleavage of ATAD-AFC by caspase-12 or related caspases, free AFC emits a yellow-green fluorescence (λmax = 505 nm) that can be quantified by using a fluorecence microtiter plate reader or a fluorometer. Comparison of the fluorescence from an apoptotic sample with an uninduced control determines the fold increase in caspase-12 activity.
