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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
β-Galactosidase in Situ Staining Kit is a kit for the detection of β-galactosidase in cells or tissues using X-gal as substrate. The principle of detection is that X-gal can produce a blue product catalyzed by β-galactosidase, and the expression of β-galactosidase can be detected by ordinary light microscopy.
This kit is optimized to be compatible with polystyrene consumables or containers such as common cell culture plates, pipettes, etc., which can effectively avoid poor staining results caused by incompatible consumables or containers. In addition, the working solution prepared by this kit cannot produce precipitation during use, which is more convenient.
Figure 1: Staining of HeLa cells transfected Plasmids expressing β-galactosidase with this kit
Components
K2182-100 T
Fixative Solution
100 mL
X-gal Solution
5 mL
Staining Solution A
1 mL
Staining Solution B
Staining Solution C
Store the kit at -20°C, stable for 1 year. X-gal Solution should be stored at -20°C away from light.