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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Cysteine-containing aspartate proteolytic enzymes (Caspase) are a family of cysteine proteases that play important roles in apoptosis, necrosis, and inflammation. Caspase-1/4/5/11 are very important in inflammatory response and pyroptosis. Caspase-4/5 can recognize and bind intracellular lipopolysaccharide (LPS), which subsequently activate inflammasomes, which in turn promote the maturation and release of inflammatory mediator IL-1β, leading to an inflammatory response. In addition, activated caspase-4/5 can also cleave the substrate protein GSDMD, triggering the formation of cell membrane pores, resulting in the release of cell contents, and ultimately pyroptosis.
Caspase-4 Fluorometric Assay Kit provides a convenient and simple way for detecting the LEVD-dependent caspase activity. LEVD-AFC (AFC:7-amino-4-trifluoromethyl coumarin) emits blue light (λmax = 400 nm); while cleavage of LEVD-AFC by Caspase-4 or related caspases, free AFC emits a yellow-green fluorescence (λmax = 505 nm), which can be quantified by using a microplate reader or a fluorometer. Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control determines the fold increase in Caspase-4 activity.
K2198-25T
K2198-100T
K2198-200T
K2198-400T
Cell Lysis Buffer
25 mL
100 mL
2X Reaction Buffer
2 mL
4 X 2 mL
16 mL
32 mL
LEVD-AFC (1 mM)
125 µL
500 µL
2X 0.5 mL
2X 1 mL
DTT (1 M)
100 µL
400 µL
Store the kit at -20°C, stable for 6 months. LEVD-AFC (1 mM) should be stored away from light.