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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Citrate is an important intermediate in the citric acid cycle which occurs in mitochondria. Citrate is produced by the addition of oxaloacetate to the acetyl group of acetyl-CoA derived from the glycolytic pathway. Citrate can also be transported out of mitochondria and transformed to acetyl CoA for fatty acid synthesis. Citrate is an allosteric modulator of both phospho-fructokinase (glycolysis) and acetyl-CoA carboxylase (fatty acid synthesis). Citrate metabolism and disposition is various due to age, sex and a variety of other factors. The Citrate Colorimetric/Fluorometric Assay Kit provides a sensitive, simple, fast and convenient way for detection of citrate levels in various biological samples based on colorimetric and fluorometric method. In the assay, citrate is converted to pyruvate via oxaloacetate. The formed pyruvate is quantified via converting a nearly colorless probe to an intensely fluorescent (Ex/Em, 535/587 nm) and colored (570 nm) product. The kit can detect 0.1 to 10 nmoles (approx. 2 µM-10 mM) citrate in various samples.