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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Lactate is chiral: L(+)-Lactate and D(-)-Lactate. L(+)-Lactate exists in blood and is constantly produced from pyruvate by lactate dehydrogenase (LDH) in human intermediary metabolism. D-Lactate is mainly produced through the glyoxalase pathway in mammals. D(-)-Lactate is present only at about 1-5% of the concentration of L(+)-Lactate and is extremely low with normal serum concentrations in the nM to µM range. The elevated D-lactate levels which can rise to mM levels are caused by short bowel syndrome or bacterial infection in humans. Abnormal high concentration of D-lactate is related to diseases such as sepsis, ischemia or trauma. Because of slow metabolism and excretion, high D-lactate can cause acidosis and encephalopathy.
The D-Lactate Colorimetric Assay Kit provides a simple, fast and convenient way for accurate detection of D-lactate levels in various biological samples (serum, plasma, cells, culture and fermentation media) based on colorimetric method. In the assay, D-lactate is specifically oxidized by D-lactate dehydrogenase and produces proportional color (λmax = 450 nm). The kit can detect the useful D-lactate concentration range 0.01 mM-10 mM in various samples.