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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Hoechst 33342/PI Double Staining Kit is a kit that can rapidly detect cell apoptosis and necrosis. This kit provides two dyes, Hoechst 33342 and propidium iodide (PI). Hoechst 33342 can penetrate cell membranes, staining normal cells with a weak blue color. Chromatin condensation is a hallmark of apoptosis. Hoechst 33342 can stain condensed chromatin of apoptotic cells more brightly than normal cells. PI cannot penetrate the cell membrane and cannot stain normal cells or apoptotic cells red; for necrotic cells (whose membrane loses integrity), PI can stain them red. When using these two dyes for double staining, normal cells are weak blue + weak red fluorescence, apoptotic cells are strong blue + weak red fluorescence, and necrotic cells are strong blue + strong red fluorescence.
Figure 1: Schematic of this kit to detect the cell apoptosis Normal cells (Control) are mainly stained with a weak blue color by Hoechst 33342; When cells are induced to undergo apoptosis, apoptotic cells (Apoptosis) are mainly stained with strong blue by Hoechst 33342, and necrotic cells are stained red by PI
Store the kit at -20°C, stable for 1 year. Hoechst 33342 staining solution and PI staining solution should be stored away from light.