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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
AO/PI Double Staining Kit is a kit that can rapidly detect cell viability. This kit provides two dyes, Acridine Orange (AO) and propidium iodide (PI). AO can penetrate cell membranes, staining normal cells in green. Chromatin condensation is a hallmark of apoptosis. AO can stain condensed chromatin of apoptotic cells more brightly than normal cells. PI cannot penetrate the cell membrane and cannot stain normal cells or apoptotic cells red; for necrotic cells (whose membrane loses integrity), PI can stain them red. The staining pattern using these two dyes makes it possible to distinguish normal, apoptotic and necrotic cells.
Figure 1: Diagram of this kit for detecting cell viability
Store the kit at -20°C, stable for 1 year. AO staining solution and PI staining solution need to be stored away from light. For frequent use, store the kit at 4°C.