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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Polybrene, also known as Hexadimethrine Bromide, is a cationic polymer widely used in retrovirus-mediated or lentivirus-mediated gene transfection. The mechanism of action may be to neutralize electrostatic repulsion between cell surface acid and viral particles. In addition, Polybrene can also be used in DNA transfection to enhance the transfection efficiency of liposomes. Meanwhile, Polybrene is an anti-heparin agent and can be used to produce non-specific agglutinated red blood cells. At the same time, Polybrene can be used in protein sequencing to enhance peptide degradation. The addition of Polybrene to the PVDF membrane can also improve the affinity of the membrane.
This product is supplied as a 10 mg/mL stock solution dissolved in 0.9% NaCl and sterilized by a 0.22 μM filter. Polybrene may be toxic to some cells, and long-term treatment (> 12 h) may also be toxic to some cells. Therefore, it is recommended to perform a toxicity test for the first time using.
This product should be stored at -20°C, and avoid repeated frozen/thaw cycles, stable for 2 years.