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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
The E. coli that can take up foreign DNA by a process called transformation are known as competent cells. In genetic engineering experiments, competent cells are often used in various DNA transformation experiments.
Super Competent Cell Preparation Kit is a kit that can quickly prepare a large number of E. coli competent cells. This kit is superior to the classical competent cell preparation kit, which is not only easy to use, but also improves the transformation efficiency of the competent cells. This kit can transform not only plasmids, but also ligation products, with a transformation efficiency of 108-109 CFU/µg for plasmids. This kit is very convenient, only needing a short time to complete the preparation after strain culture. This kit is suitable for most common E. coli strains such as DH5α, JM109, TG1, HB101 and XL-1. It is also suitable for some E. coli strains used for protein expression or viral plasmid construction, but sometimes it is not as efficient as other common strains when used for transformation of ligation products.
This kit provides the ready-to-use Super Competent Culture Medium, which can improve the transformation efficiency of competent cells.
This product should be stored at -20°C, avoiding the repeated freeze/thaw cycles, and stable for 1 year.