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Name
HyperFluor™ 405 Goat Anti-Human IgG (H+L) Antibody
Host species
Goat
Target species
Human
Tested applications
ICC/IF,IHC-Fr,IHC-P,ICC,Flow Cyt,ELISA
Conjugation
HyperFluor™ 405
Ex/Em
403 nm / 427 nm
Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for Human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g., HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e., immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
Application
Notes
ICC/IF
1/500 - 1/2000
IHC-Fr
Use at an assay dependent dilution.
IHC-P
1/100 - 1/500
Flow Cyt
1/250 - 1/1000
ELISA
Form
Liquid
Storage buffer
50% Glycerol,1% BSA,PBS,0.02% NaN3
Storage instructions
Shipped at 4°C. Store at 4°C short term (2 weeks). Upon delivery aliquot. Store at -20°C.
Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Keep away from Light.
Concentration
1 mg/mL
Purity
Immunogen affinity purified
Purification notes
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
Clonality
Polyclonal
lsotype
IgG