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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
BCA stands for Bicinchoninic Acid Assay, which is a protein quantification method based on the biuret principle. Under alkaline conditions, the protein peptide bond reduces Cu2+ in copper sulfate to Cu+, and then BCA chelates Cu+ to form a purple complex that strongly absorbs light at a wavelength of 562 nm. The color of the chelate is related to the protein concentration, and the amount of Cu2+ or Cu+ obtained is proportional to the amount of protein present in the solution, so the protein concentration to be measured can be obtained by measuring the protein absorbance value.
This kit is designed for protein quantification of low protein concentration samples and can measure the total protein concentration of diluted protein solution (0.5~20 μg/mL). This kit has the characteristics of simple operation, high stability, high sensitivity, and high compatibility, and has good compatibility with a variety of ionic and non-ionic detergents.
Store Reagent B at 4°C away from light and the other components at 4°C for a year.