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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Diaminobenzidine (3,3'-diaminobenzidine, DAB) is a common chromogenic substrate of horseradish peroxidase (HRP), as an HRP electron donor, loses electrons in the presence of hydrogen peroxide, rapidly changes color, and forms insoluble brown or amber particles through polymerization and other reactions, and settles in situ. This brown precipitate is insoluble in water and ethanol, so after DAB color development, subsequent staining can also be performed using an ethanol-soluble dye.
This kit is a horseradish peroxidase (HRP) substrate detection kit that can be used for immunohistochemistry, in situ hybridisation, protein blotting, spot blotting and other experiments.
Store the components at -20°C away from light for 12 months.