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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Cell lines
The human cholangiocarcinoma (CCA) cell lines HCCC9810 and QBC939
Preparation method
The solubility of this compound in DMSO is >12.3mg/mL. General tips for obtaining a higher concentration: Please warm the tube at 37℃ for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below -20℃ for several months.
Reacting condition
0, 30, 60, 90, 120, or 150 μM; 24, 48, or 72 h; 37°C
Applications
In CCA cells, kaempferol significantly inhibited the viability and proliferation of CCA cells in a time- and dose-dependent way. Kaempferol reduced the number of colonies and induced apoptosis in a dose-dependent way. Kaempferol also reduced the migration and invasion ability of CCA cells in a dose-dependent manner.
Animal models
subcutaneous xenograft nude mice model
Dosage form
20 mg/kg/day; daily for 3 weeks; i.p.
Application
In subcutaneous xenograft nude mice model, kaempferol significantly inhibited the growth of the tumor. The number of Ki-67-positive tumor cells was lower in kaempferol-treated group than that in the control group. Kaempferol reduced the average volume and number of foci per mouse.
Other notes
Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal.
References:
[1]. Qin Y1, Cui W2, Yang X2, et al. Kaempferol inhibits the growth and metastasis of cholangiocarcinoma in vitro and in vivo. Acta Biochim Biophys Sin (Shanghai). 2016 Mar;48(3):238-45.