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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Cell lines
pcDNA3-FADDdn-transfected BJAB cells
Preparation method
The solubility of this compound in DMSO is >12.2mg/mL. General tips for obtaining a higher concentration: Please warm the tube at 37℃ for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below -20℃ for several months.
Reacting condition
15 to 100 μM for 36 h.
Applications
Piceatannol-induced apoptosis in BJAB cells was mediated by loss of mitochondrial membrane. Piceatannol led to a significant loss of the mitochondrial membrane potential at relatively low concentrations of 15 and 25μM. Piceatannol at concentrations < 100μM significantly did not reduce viability of BJAB cells thereby indicating that a membrane disrupting effect of this naturally occurring polyhydroxystilbenes, ie unspecific necrosis, did not play a role for their death-inducing potency. Apoptosis induction was concentration-dependent with a half-maximum concentration of 25μM for piceatannol.
Animal models
Six-week-old female BALB/c mice
Dosage form
1, 2.5, 5, or 10 mg/kg of body weight by gavage for 7 days
Application
The DAI (disease activity index) decreased significantly in the mice receiving piceatannol (2.5–10 mg/kg) compared with the mice receiving vehicle treatment. And piceatannol (2.5–10 mg/kg) treatment reduced weight loss in mice with colitis. NO and PGE2 are considered important inflammatory mediators, playing a key role in the pathogenesis of IBD (Inflammatory bowel disease). Oral administration of piceatannol reduced NO and PGE2 production in a concentration-dependent manner at day 10. Piceatannol administration (10 mg/kg) prevented significant increases in IL-1β, IL-6, and TNF-α levels. Administration of piceatannol 10 mg/kg significantly decreased the translocation of phospho-STAT3 and of the p65 subunit of NF-κB to enterocyte nuclei. Thus 10 mg/kg of piceatannol dramatically reduced MCP-1 and KC production in the colon. This indicated that piceatannol exerted anti-inflammatory effects by reducing monocyte and neutrophil infiltration into the colonic mucosa.
Other notes
Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal.
References:
[1].Wieder, T.,Prokop, A.,Bagci, B., et al. Piceatannol, a hydroxylated analog of the chemopreventive agent resveratrol, is a potent inducer of apoptosis in the lymphoma cell line BJAB and in primary, leukemic lymphoblasts. Leukemia 15, 1735-1742.
[2]. Kim YH1, Kwon HS, Kim DH., et al. Piceatannol, a stilbene present in grapes, attenuates dextran sulfate sodium-induced colitis. Int Immunopharmacol. 2008 Dec 10;8(12):1695-702.
