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Recombinant Human Interleukin-36 Receptor Antagonist Protein

Recombinant Human Interleukin-36 Receptor Antagonist Protein

Catalog No.

P1261

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5ug	$99.00	Ship with 5-10 days
100ug	$813.00	Ship with 5-10 days
500ug	$1,825.00	Ship with 5-10 days

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Background
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Background

Interleukin-36 receptor antagonist (IL-36RA) is a secreted protein which belongs to the interleukin 1 cytokine family (IL-1 family) and it is predominantly expressed in keratinocytes but not in fibroblasts, endothelial cells or melanocytes. IL-36RA has been reported to antagonize the biological activity of IL-36α (IL-1F6), IL-36β (IL-1F8), and IL-36γ (IL-1F9). Furthermore, it could constitute part of an independent signaling system analogous to interleukin-1 alpha (IL-1A), beta (IL-1B) receptor agonist and interleukin-1 receptor type I (IL-1R1). In addition, The receptor for IL-36RA has not been positively identified. Indirect evidence suggests it is IL-1Rrp2. Recombinant human IL-36 RA contains 154 amino acid residues and it shares 91 % a.a. sequence identity with murine IL-36RA.

Reference

1. Born TL, Smith DE, Garka KE, et al. 2000. J Biol Chem. 275:41528

2. Dunn EF, Gay NJ, Bristow AF, et al. 2003. Biochemistry. 42:10938-44

3. Barton JL, Herbst R, Bosisio D, et al. 2000. Eur J Immunol. 30:3299-308

4. Debets R, Timans JC, Homey B, et al. 2001. J Immunol. 167:1440-6

5. Towne JE, Renshaw BR, Douangpanya J, et al. 2011. J Biol Chem. 286:42594-602.

Information

Gene ID	26525
Accession #	Q9UBH0
Alternate Names	IL-1RP3, IL-1HY1, IL-1 delta, IL-1F5, IL-1ra homolog 1, IL-1L1
Source	Escherichia coli.
M.Wt	Approximately 16.8 kDa, a single non-glycosylated polypeptide chain containing 154 amino acids.
AA Sequence	VLSGALCFRM KDSALKVLYL HNNQLLAGGL HAGKVIKGEE ISVVPNRWLD ASLSPVILGV QGGSQCLSCG VGQEPTLTLE PVNIMELYLG AKESKSFTFY RRDMGLTSSF ESAAYPGWFL CTVPEADQPV RLTQLPENGG WNAPITDFYF QQCD
Appearance	Sterile Filtered White lyophilized (freeze-dried) powder.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied. - 1 month, 2 to 8 °C under sterile conditions after reconstitution. - 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Formulation	Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Biological Activity	Fully biologically active when compared to standard. The ED₅₀ as determined by inhibiting IL-36 beta induced IL-8 secretion by human preadipocytes is less than 1.0 μg/ml, corresponding to a specific activity of > 1000 IU/mg in the presence of 20 ng/ml of recombinant human IL-36 beta.
Shipping Condition	Gel pack.
Handling	Centrifuge the vial prior to opening.
Usage	For Research Use Only! Not to be used in humans.

Quality Control

Quality Control & DataSheet

View current batch:

Purity > 95 % by SDS-PAGE and HPLC analyses.
Endotoxin: Less than 1 EU/μg of rHuIL-36RA as determined by LAL method.

Datasheet

Recombinant Human Interleukin-36 Receptor Antagonist Protein

mRNA synthesis

Tyramide Signal Amplification (TSA)

Phos Binding Reagent Acrylamide

Cell Counting Kit-8 (CCK-8)

Inhibitor Cocktails

Background

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Quality Control & DataSheet

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