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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
HyperChrom AR rProtein A FF Agarose is an affinity chromatography medium for antibody purification, separated by a specific interaction between antigen and antibody. HyperTrap AR rProtein A FF Column Ready-to-use chromatography column with HyperChrom AR rProtein A FF Agarose as the packing is mainly used for the purification of monoclonal and polyclonal antibodies, with the advantages of high flow rate, high load, easy regeneration, low cost and easy amplification.
The main body and inner plug of the pre-installed column tube are made of PP, and the inside and outside are polished, and the sieve plate is made of HDPE material, which has excellent physical properties and chemical resistance, corrosion resistance, anti-aging, long life and good safety. 1) Ready-to-use, convenient and fast. 2) Can be used with syringes, peristaltic pumps or chromatography systems. 3) It can be used in series to increase the sample processing capacity.
1. HyperTrap AR rProtein A FF Column preloaded column parameters
Pre-assembled column specification/name
1 mL
5 mL
Dimensions (IDxH)
7.7 x 25 mm
16 x 25 mm
Withstand pressure
0.3 MPa
Workflow Speed*
1 mL/min
1-3 mL/min
Operating temperature
4 - 30°C
Storage condition
Store the components at 4℃ for 5 years.
*This flow rate is the recommended common flow rate, the pressure resistance, and the use of different types of chromatography packing in the pre-assembled column will be different, please refer to the product manual of the corresponding chromatographic filler.
2. HyperTrap AR rProtein A FF Column chromatography media parameters
Name
Description
Chromatography media type
Affinity chromatography media
Ligation
rProtein A (E. coli recombinant expression)
Scaffolding
Highly cross-linked agarose
Average particle size
90 μm
Ligand density
6 mg rProtein A/ml chromatography medium
Dynamic load
~35 mg human IgG/mL chromatography medium*
Flow rates are recommended
150-500 cm/h
Maximum flow rate
700 cm/h
Use temperature
4-30℃
pH stability **
3-10
Chemical resistance
6 M guanidine hydrochloride, 8 M urea, 2% benzyl alcohol, 75% ethanol, 1 mM NaOH (pH11), 0.1 M sodium citrate/HCl (pH3).
** Dynamic load measurement conditions: column loading height: 5 cm, retention time 3 min, test buffer: 0.02 M NaH2PO4 solution, pH 7.0, test sample: human lgG sample, lgG sample per unit media volume (ml) when the penetration of lgG reaches 10%.
**After 7 days of storage of chromatography medium at 40°C and pH 3-10, its physicochemical properties and functions did not change significantly.
***30% and 75% are v/v, volume ratio.