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HyperTrap AR rProtein A/G 4FF Column is an affinity chromatography z-packed column for antibody purification that is separated by a specific interaction between antigen and antibody, mainly for the purification of IgG in culture fluid, serum, ascites, or hybridoma cell culture supernatant, or the purification of recombinant proteins containing Fc fragments.
HyperChrom AR rProtein A FF Agarose and HyperChrom Protein G 4FF Agarose in this product are covalently connected to 4% high crosslinked, high flow rate agarose and mixed in a 1:1 ratio. Each milliliter of HyperChrom AR rProtein A/G Agarose 4FF Agoros can bind approximately 20 mg of human IgG.
1. HyperTrap AR rProtein A/G 4FF Column preloaded column parameters
Pre-assembled column specification/name
1 mL
5 mL
Dimensions (IDxH)
7.7 x 25 mm
16 x 25 mm
Withstand pressure
0.3 MPa
Workflow Speed*
1 mL/min
1-3 mL/min
Operating temperature
4 - 30°C
Storage condition
Store the components at 4℃ for 5 years.
*This flow rate is the recommended common flow rate, the pressure resistance, and the use of different types of chromatography packing in the pre-assembled column will be different, please refer to the product manual of the corresponding chromatographic filler.
2. HyperTrap AR rProtein A/G 4FF Column chromatography media parameters
Name
Description
Chromatography media type
Affinity chromatography media
Ligation
rProtein A/G (E. coli recombinant expression, 1:1).
Scaffolding
Highly cross-linked agarose
Average particle size
90 μm
Dynamic load
~20 mg human IgG/mL chromatography medium
Flow rates are recommended
50-400 cm/h
Use temperature
4-30℃
pH stability
3-9