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HyperChrom Glutathione 4FF Agarose is an affinity chromatography medium primarily used to purify glutathione S-transferase (GST)-tagged proteins, but also for the purification of other S-transferases or glutathione-dependent proteins. HyperChrom Glutathione 4FF Agarose is the first choice for purifying GST-tagged proteins, with the advantages of high loading, easy regeneration, and easy amplification.
HyperChrom Glutathione 4FF Agarose chromatography media parameters
Name
Description
Chromatography media type
Affinity chromatography media
Ligation
Glutathione and 10 carbon atoms connect arms
Scaffolding
4% highly cross-linked agarose
Average particle size
90 μm
Ligand density
120~320 μmol glutathione/mL chromatography medium
Dynamic load
≥ 10 mg GST-tagged protein/mL chromatography medium
Flow rates are recommended
90-300 cm/h
Maximum flow rate
400 cm/h
Withstand pressure
0.3 MPa
Use temperature
4 - 30℃
pH stability *
3 - 12
Chemical resistance
Common aqueous solution, 0.01 M HCl, 0.1 M NaOH, 8 M urea, 6 M guanidine hydrochloride, 30% isopropanol **
* After the chromatography medium was placed at 40 °C and pH 3-12 for 7 days, its physicochemical properties and functions did not change significantly.
** 30% is v/v, volume ratio.
Store the components at 4℃ for 5 years.