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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
HyperChrom Resilient ARPA Agarose is an affinity chromatography medium for antibody purification that is separated by a specific interaction between antigen and antibody. This chromatography medium is used to process high concentrations of monoclonal antibodies.
HyperChrom Resilient ARPA Agarose has the characteristics of high load, high flow rate and alkali resistance: (1) High flow rate and high dynamic binding load shorten the processing time. (2) The modified alkali-resistant rProtein A ligand can withstand 0.5M NaOH for CIP.
HyperChrom Resilient ARPA Agarose chromatography media parameters
Name
Description
Chromatography media type
Affinity chromatography media
Ligation
Alkali resistant Protein A
Scaffolding
Highly cross-linked agarose
Average particle size
85 μm
Dynamic load
≥ 60 mg human IgG/mL chromatography medium*
Flow rates are recommended
90-500 cm/h
Maximum flow rate
700 cm/h
Withstand pressure
0.3 MPa
Use temperature
2-40℃
pH stability **
2-14
Chemical resistance
A water-soluble buffer commonly used in Protein A chromatography
*Dynamic load measurement conditions: column loading height: 10 cm, retention time 6 min, test buffer: 0.02 M NaH2PO4 solution, 0.15 M NaCl, pH 7.4, lgG loading volume per unit media volume (mL) (mg) when lgG penetration reaches 10%.
**After 7 days of storage of chromatography medium at 40 °C and pH 2-14, its physicochemical properties and functions did not change significantly.
Store the components at 4℃ for 5 years.