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HyperChrom Protein G 4FF Agarose is an affinity chromatography medium made by coupling Protein G to the Chromstar substrate by CNBr activation. Protein G can bind to the Fc region of a variety of mammalian IgG and has a weak affinity for its Fab region.
Compared to Protein A, Protein G has a broader binding spectrum. Therefore, HyperChrom Protein G 4FF Agarose can be used to isolate and purify immunoglobulins and their fragments from different liquid samples such as cell culture media or serum. HyperChrom Protein G 4FF Agarose can also be used to isolate immune complexes.
HyperChrom Protein G 4FF Agarose chromatography media parameters
Name
Description
Chromatography media type
Affinity chromatography media
Ligation
Protein G (E. coli recombinant expression).
Scaffolding
Highly cross-linked agarose
Average particle size
90 μm
Ligand density
~2 mg Protein G/mL chromatography medium
Dynamic load
≥ 20 mg human IgG/mL chromatography medium*
Flow rates are recommended
90-300 cm/h
Maximum flow rate
400 cm/h
Withstand pressure
0.3 MPa
Use temperature
4-30℃
pH stability **
3-10
Chemical resistance
Common aqueous solution, 30% isopropanol*** 75% ethanol*** 6 M guanidine hydrochloride, 8 M urea
*Dynamic load measurement conditions: column loading height: 10 cm, test flow rate 200 cm/h, test buffer: 0.02 M NaH2PO4 solution, pH 7.0, test sample: 2 mg/m IgG sample, when the penetration of IgG reaches 10%, unit medium volume (mL) IgG loading volume (mg).
**After 7 days of storage in the environment of 40°C and pH 3-9, the physicochemical properties and functions of the chromatography medium did not change significantly.
*** 30% and 75% are the volume ratio (v/v).
Store the components at 4℃ for 5 years.