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HyperChrom Phenyl 6FF (HS) Agoros is a class of hydrophobic interaction chromatography media that takes advantage of differences in the hydrophobic properties and the amount of different molecules under specific conditions. This chromatography medium has a high degree of ligand substitution and is mainly used for the isolation and purification of a variety of biomolecules, such as recombinant proteins, antibodies, viral vaccines, etc.
The HyperChrom Phenyl 6FF (HS) Agoros series offers excellent scale-up performance: (1) Highly crosslinked agarose base with excellent rigidity, resulting in high process flow rates and improved process efficiency at low backpressure. (2) Hydrophilic base frame to minimize the influence on the hydrophobic role of the ligand. (3) Through chemical modification, it has excellent chemical compatibility and is resistant to CIP cleaning such as sodium hydroxide.
HyperChrom Phenyl 6FF (HS) Agarose chromatography media parameters
Name
Description
Chromatography media type
Hydrophobic interactions
Ligation
R-O-CH2-CH(OH)-CH2-O-C6H5
Scaffolding
Highly cross-linked agarose
Average particle size
90 μm
Ligand density
45 μmol phenyl group/mL chromatography medium (HS)
Flow rates are recommended
150-300 cm/h
Maximum flow rate
600 cm/h
Withstand pressure
0.3 MPa
Use temperature
4-30℃
pH stability*
2-14
Solvent resistant
Common aqueous solution, 3 M ammonium sulfate, 30% isopropanol**, 75% ethanol**, 10% ethylene glycol**, 1 M NaOH, 1 M acetic acid, 6 M guanidine hydrochloride, 8 M urea
Intolerant to solvents
Oxidant
* After 7 days of storage of chromatography medium at 40°C and pH 2-14 environment, its physicochemical properties and functions did not change significantly.
**30%, 75% and 10% are v/v, volume ratio.
Store the components at 4℃ for 5 years.