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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Oncostatin M (OSM) is a glycoprotein belonging to the interleukin-6 family of cytokines that has functions mainly in cell growth. Oncostatin M (OSM) is considered as a pleiotropic cytokine that signals through cell surface receptors typeⅠ and typeⅡ both of which share the similarity of containing protein gp130 and takes part in many bio metabolism processes including liver development, hematopoiesis, inflammation, bone formation, and destruction and possibly CNS development. Oncostatin M (OSM) was previously identified by its ability to inhibit the growth of cells from melanoma and other solid tumors. It also has been reported that OSM, like LIF, IL-6, and G-CSF, can inhibit the proliferation of murine M1 myeloid leukemic cells and can induce their differentiation into macrophage-like cells. The human form of OSM is insensitive between pH2 and 11 and resistant to heating for one hour at 56 degrees but is not stable at 90 degrees. The human OSM is produced as a precursor containing 252 amino acids, whose first 25 amino acids function as a secretory signal peptide and which on removal yields the soluble 227 amino acid pro-OSM. Removal of the C-terminal most 31 amino acids produce the fully active 196 residue form.
Reference
[1]. Tanaka M, et al. (2003) Rev Physiol Biochem Pharmacol. 149: 39-52.
[2]. Auguste P, et al. (1997) J Biol Chem. 272 (25): 15760-4.
[3]. Zarling JM, et al. (1986) Proc Natl Acad Sci. 83 (24): 9739-43.
Accession #
P13725
Alternate Names
MGC20461; oncostatin M; oncostatin-M; OSM
Source
Human embryonic kidney cell, HEK293-derived human Oncostatin M/OSM protein
Protein sequence
Ala26-Arg221
M.Wt
25.8 kDa
Appearance
Solution protein.
Stability & Storage
Avoid repeated freeze-thaw cycles. It is recommended that the protein be aliquoted for optimal storage. 12 months from date of receipt, -20 to -70 °C as supplied.
Concentration
0. 2 mg/mL
Formulation
Dissolved in sterile PBS buffer.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. This solution can be diluted into other aqueous buffers.
Biological Activity
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The EC50 for this effect is 5-30 pg/mL.
Shipping Condition
Shipping with dry ice.
Handling
Centrifuge the vial prior to opening.
Usage
For Research Use Only! Not to be used in humans.