10Panx
10Panx is a mimetic inhibitory peptide of panx1, which is a mammalian protein ectopically expressed to function as a hemi-channel, that readily and reversibly inhibits the panx1 currents. 10Panx has attracted increasing attention as it allows scientists readily and reliably to examine inhibition of panx1 in cells where efficient and consistent delivery of siRNA is highly difficult. According to results of multiple previous studies, 10Panx selectively inhibits P2X7R-induced dye uptake without altering other aspects of R2X7R activation and ATP-evoked membrane currents, constitutive dye-uptake and hemichannel-like currents that are induced by the overexpression of panx1, and ATP-mediated IL-1β release.
Reference
Pablo Pelegrin and Annmarie Surprenant. Pannexin-1 mediates large pore formation and interleukin-1β release by the ATP-gated P2X7 receptor. The EMBO Journal (2006) 25, 5071-5082
- 1. PusenWang, Baojie Shi1, et al. "Hepatic pannexin‐1 mediates ST2+ regulatory T cells promoting resolution of inflammation in lipopolysaccharide‐induced endotoxemia." Clin Transl Med. 2022 May;12(5):e849. PMID: 35593197
- 2. Shuzhen Dong, Kai Zhang, et al. "Carbenoxolone has the potential to ameliorate acute incision pain in rats." Mol Med Rep. 2021 Jul;24(1):520. PMID: 34013377
- 3. Fan J, Chen Y, et al. "Multi-walled carbon nanotubes induce IL-1β secretion by activating hemichannels-mediated ATP release in THP-1 macrophages." Nanotoxicology. 2020;1-18. PMID: 32538272
- 4. Wang Q, Li M, et al. "Mitochondrial Protein UQCRC1 is Oncogenic and a Potential Therapeutic Target for Pancreatic Cancer." Theranostics. 2020;10(5):2141–2157. PMID: 32089737
- 5. Gao J, Zhang T, et al. "The F0F1 ATP synthase regulates human neutrophil migration through cytoplasmic proton extrusion coupled with ATP generation." Mol Immunol. 2017 Aug 23;90:219-226. PMID: 28843171
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 1242.37 |
| Cas No. | 955091-53-9 |
| Formula | C58H79N15O16 |
| Synonyms | 10Panx |
| Solubility | ≥124.2 mg/mL in DMSO; insoluble in EtOH; ≥4.26 mg/mL in H2O with ultrasonic |
| SDF | Download SDF |
| Canonical SMILES | CC(C)C(C(=O)NC(CC(=O)O)C(=O)NC(CO)C(=O)NC(CC1=CC=C(C=C1)O)C(=O)O)NC(=O)C(CC2=CC=CC=C2)NC(=O)C(C)NC(=O)C(C)NC(=O)C(CCC(=O)N)NC(=O)C(CCCN=C(N)N)NC(=O)C(CC3=CNC4=CC=CC=C43)N |
| Shipping Condition | Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice. |
| General tips | We do not recommend long-term storage for the solution, please use it up soon. |
| Cell experiment: [1] | |
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Cell lines |
Human red blood cells (RBCs) |
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Preparation method |
The solubility of this peptide in sterile water is >10 mM. Stock solution should be splited and stored at -80°C for several months. |
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Reaction Conditions |
200 μM, |
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Applications |
10Panx reduced ATP release in response to lowered pO2 by 90.9±15.5%. It did not reduce ILO-induced ATP release. |
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Other notes |
Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal. |
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References: [1] Sridharan M, Adderley S, Bowles E, et al. Pannexin 1 (PANX1) is a Conduit for ATP Released from Human Red Blood Cells (RBCs) Exposed to Low Oxygen Tension. The FASEB Journal, 2010. |
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| Description | 10Panx, Panx-1 mimetic inhibitory peptide, is a blocker of pannexin-1 gap junctions. | |||||
| Targets | Gap Channels | |||||
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Quality Control & MSDS
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Chemical structure
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