Single Firefly Luciferase Reporter Gene Assay Kit II

Firefly luciferase is a protein with a molecular weight of approximately 61 kD, which catalyzes the oxidation of the substrate luciferin to oxyluciferin in the presence of ATP, oxygen, and magnesium ions. During the oxidation of luciferin, a bioluminescence with a wavelength of about 560 nm is emitted, which can be measured by a luminometer or a liquid flash meter. The bioluminescence system of luciferin and luciferase allows for very sensitive and efficient detection of gene expression. Usually, the transcriptional regulatory element or 5' promoter region of the gene of interest is cloned upstream of luciferase, or the 3'-UTR region is cloned downstream of luciferase, etc., to construct a reporter gene plasmid, transfect the cells, and treat the cells with appropriate drugs, and finally measure the luciferase activity in the cell lysate and judge the transcriptional regulation effect of the drug treatment on the target gene by the level of luciferase activity.

Single Firefly Luciferase Reporter Gene Assay Kit II is a high-sensitivity, high-signal robust, one-step assay for the direct determination of intracellular firefly luciferase activity by chemiluminescence without the need to wash or collect cells.

This product is a different packaging version of the Firefly Luciferase Reporter Gene Assay Kit (K2236), and the detection results of both are identical. K2236 is a ready-to-use reagent, the advantage is that it can be used directly without preparation, and the disadvantage is that it needs to be stored at -80ºC, and the detection effect will gradually decrease after a long storage time at -20ºC. This product is a lyophilized powder version of K2236 that needs to be fully dissolved before use, but it can be stored at -20ºC stably.