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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
APExBIO Streptavidin Beads C3 are ideal for nucleic acid applications (specifically for samples with a high chaotropic salt concentration), immunoassays involving small biotinylated antigens and applications that are not compatible with bovine serum albumin (BSA).
Add the beads to a sample containing biotinylated molecules, e.g. peptides or oligonucleotides. During a short incubation, the biotinylated molecule binds to the beads. The molecule-bead complex can be separated by using a magnet. Capture, washing, and detection can be optimized for manual or automated use. With indirect target capture, mix the biotinylated molecule with the sample to capture the molecule-target complex before adding the beads.
CAUTION: Sodium azide may react with lead and copper plumbing to form highly explosive metal azides.
Hydrophilic bead surface
Based on carboxylic acid beads
No blocking proteins used
Isoelectric point: pH 4.5
High charge (–50 mV, at pH 7)
Iron content (ferrites): 14% (20%)
Low aggregation of beads in high-salt solutions