Streptavidin Beads C3
APExBIO Streptavidin Beads C3 are ideal for nucleic acid applications (specifically for samples with a high chaotropic salt concentration), immunoassays involving small biotinylated antigens and applications that are not compatible with bovine serum albumin (BSA).
Add the beads to a sample containing biotinylated molecules, e.g. peptides or oligonucleotides. During a short incubation, the biotinylated molecule binds to the beads. The molecule-bead complex can be separated by using a magnet. Capture, washing, and detection can be optimized for manual or automated use. With indirect target capture, mix the biotinylated molecule with the sample to capture the molecule-target complex before adding the beads.
| Name | V (mL) | Concentration | Supplied in | Bead diameter (μm) | Storage | Recommended protein binding amount |
| Streptavidin Beads C3 | 1 or 5*1 mL | 10 mg/mL | PBS, pH 7.4 / 0.14 M NaCl / 0.02% NaN3 |
3 | Store at 2-8˚C | 10 μg IgG/mg |
| *Note: APExBIO M-270 Streptavidin supplied in 0.0065 M phosphate buffer pH 7.4, with 0.14 M NaCl and 0.02% sodium azide as a preservative. | ||||||
CAUTION: Sodium azide may react with lead and copper plumbing to form highly explosive metal azides.
Hydrophilic bead surface
Based on carboxylic acid beads
No blocking proteins used
Isoelectric point: pH 4.5
High charge (–50 mV, at pH 7)
Iron content (ferrites): 14% (20%)
Low aggregation of beads in high-salt solutions
