Streptavidin -HRP
Streptavidin is a protein that has similar binding properties to avidin. It is isolated from Streptomyces Avidinii. Streptavidin has a molecular weight of 60 KD and has 4 subunits. Each subunit can bind one molecule of biotin. Biotin is a 244 dalton water-soluble vitamin. Streptavidin has an extremely high binding affinity (Kd=10-15) for biotin. Streptavidin peroxidase is used in a labeled streptavidin-biotin immunoenzymatic antigen detection system. This technique involves the sequential incubation of the specimen with an unconjugated primary antibody specific to the target antigen, a biotinylated secondary antibody which reacts with the primary antibody, enzyme-labeled streptavidin, and substrate-chromogen. This conjugate also binds to nucleic acids labeled with biotinylated nucleotide analogs in Southern or Northern blot.
| Application | Notes |
| Dot blot | Use at an assay dependent dilution. |
| ELISA | Use at an assay dependent dilution. |
| IHC-P | Use at an assay dependent dilution. |
| IHC-Fr | Use at an assay dependent dilution. |
| Immunomicroscopy | Use at an assay dependent dilution. |
| ICC | Use at an assay dependent dilution. |
| WB | 1/2000-1/20000. |
| Form | Liquid |
| Storage buffer | pH: 7.40 Preservative: 1% BSA,50% glycerol,0.01% proclin 300 Constituent: PBS |
| Storage instructions | Shipped at 4°C. Store at 4°C short term (2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. |
| Concentration | 1 mg/mL |