T4 DNA Ligase (RNase-free)
Catalog No.
K1100
T4 DNA ligase, fragments ligation for cohesive or blunt end, nicks repair
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T4 DNA Ligase is a single-stranded polypeptide with a molecular weight of 55.3 kDa, which can catalyze the formation of phosphodiester bonds at adjacent 5’ phosphate and 3’ hydroxyl ends on double-stranded DNA or RNA. The ligase has no catalytic activity for single-stranded nucleic acids, and its catalytic reaction requires ATP as a cofactor.
T4 DNA ligase is versatile, can not only bind DNA fragments to cohesive or blunt ends, but also repair single-stranded nicks on double-stranded DNA, double-stranded RNA, or DNA/RNA hybrids. It is commonly used for connection of DNA fragments with vectors, linkers or adaptors; nicks repair; and linear DNA self-cyclization.
| Components | 40 KU | 100 KU | 200 KU |
| T4 DNA Ligase (400 U/μL) | 0.1 mL | 0.25 mL | 0.5 mL |
| 10X T4 DNA Ligase Reaction Buffer | 1 mL | 2.5 mL | 5 mL |
Store the components at -20 °C. | |||