T7 RNA Polymerase
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- 2. Yue Wang, Yatu Peng, et al. "Co-delivery of Cas9 mRNA and guide RNAs for editing of LGMN gene represses breast cancer cell metastasis." Sci Rep. 2024 Apr 6;14(1):8095. PMID: 38582932
This product is an E. coli recombinant expressed phage T7 RNA polymerase, a DNA-dependent RNA polymerase with high specificity for the T7 phage promoter. This 99 KD T7 RNA polymerase uses double-stranded DNA containing the T7 promoter sequence as a template and NTP as a substrate to synthesize RNA complementary to the reverse single-stranded DNA downstream of the promoter. Double-stranded linear blunt-end or 5'sticky-end DNA can be used as a substrate template for T7 RNA polymerase, so linear plasmids and PCR products can be used as templates for in vitro synthesis of RNA.
RNA synthesized by T7 RNA polymerase covers a wide range of applications, such as in vitro translation, antisense RNA and RNAi experiments, RNA vaccines, RNA structure and function studies, ribozyme biochemistry, RNase protein experiments and probe-based hybridization blots.
| Components | 5000 U | 25000 U | 100000 U |
| T7 RNA Polymerase (50 U/μL) | 100 μL | 500 μL | 2 mL |
| 10X Reaction Buffer | 100 μL | 500 μL | 2 mL |
Store all the kit components at -20°C. | |||