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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Taq DNA Ligase is a thermostable ligase that catalyzes the formation of phosphodiester bonds between the 5′-phosphate and 3′-hydroxyl groups of two adjacent oligonucleotide chains that hybridize to the same complementary target DNA strand. This reaction occurs only when the two oligonucleotide chains are perfectly paired with the target DNA with no gaps, making it suitable for single-base substitution detection. Taq DNA Ligase uses NAD+ as a cofactor and remains active within a temperature range of 45–65℃.
Components
K3153-1000U
Storage
Taq DNA Ligase
25 μL
-20℃
10× Taq DNA Ligase Reaction Buffer
1 mL