TCEP hydrochloride
Tris (2-carboxyethyl) phosphine hydrochloride (TCEP HCL) is a water soluble strong reducing agent that cleave disulfide bonds. It is a non-thiol and non-volatile solid. It can be utizlied as catalyst for the reduction of sulfonyl chlorides, N-oxides, sulfoxides and azides.
In measuring DHA (Dehydroascorbic acid) content in multiple tissues and plasma of 6-weeks-old mice, 35 mM TCEP HCL fully reduced DHA to AA (Ascorbic acid) after 2 hours on ice in a 5% solution of metaphosphoric acid containing 1 mM ethylenediaminetetraacetic acid (EDTA) at pH 1.5 [1]. In Aspergillus saitoi protease type XIII and porcine pepsin, TCEP can also be used in proteolysis and hydrogen/deuterium exchange [2].
Reference:
1. Sato Y, Uchiki T, Iwama M, Kishimoto Y et al. Determination of dehydroascorbic acid in mouse tissues and plasma by using tris(2-carboxyethyl)phosphine hydrochloride as reductant in metaphosphoric acid/ethylenediaminetetraacetic acid solution. Biol Pharm Bull. 2010;33(3):364-9.
2. Zhang HM, McLoughlin SM, Frausto SD et al. Simultaneous reduction and digestion of proteins with disulfide bonds for hydrogen/deuterium exchange monitored by mass spectrometry. Anal Chem. 2010 Feb 15;82(4):1450-4.
- 1. Chapman Ho, Clíona McMahon, et al. "Triggered ‘capture-and-release’ enables a high-affinity re-binding strategy for sensitivity enhancement in lateral flow assays." Chem Rxiv. 10 January 2025, Version 1
- 2. Wei Song, Yichen Zhao, et al. "The dual ubiquitin binding mode of SPRTN secures rapid spatiotemporal proteolysis of DNA-protein crosslinks." bioRxiv. November 26, 2024.
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 286.65 |
| Cas No. | 51805-45-9 |
| Formula | C9H16ClO6P |
| Solubility | ≥28.7 mg/mL in H2O; insoluble in EtOH; ≥25.7 mg/mL in DMSO |
| Chemical Name | 3,3',3''-phosphinetriyltripropanoic acid hydrochloride |
| SDF | Download SDF |
| Canonical SMILES | O=C(O)CCP(CCC(O)=O)CCC(O)=O.Cl |
| Shipping Condition | Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice. |
| General tips | We do not recommend long-term storage for the solution, please use it up soon. |
Quality Control & MSDS
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Chemical structure
