TSA Multi-colour Immunofluorescence System Kit (4-Color)

Tyramide Signal Amplification (TSA) technology can be used to detect low-abundance targets in tissue and cell immunofluorescence (IF), immunochemistry, in situ hybridization (ISH), etc., increasing signal sensitivity by about 100-fold. The HRP-labeled secondary antibody can activate the fluorescently labeled tyramide molecules in the presence of H2O2, and convert the labeled tyramide into an oxidized, highly reactive free radical that can covalently bind to tyrosine residues in the vicinity of the reaction site. The labeling process is rapid (less than 10 minutes), and the deposition marks can be viewed directly under a standard or confocal microscope. TSA fluorescence kits can also be used in combination with traditional immunofluorescence methods for multicolor imaging, or two or more tyramide reactions can be performed sequentially to label different targets on one sample (for example, in this kit, three different fluorescent tyramide molecules were employed). The use of TSA reagents can significantly improve signal sensitivity while maintaining the specificity and resolution ratio compared to normal experiments, in addition, TSA reagents can significantly reduce the consumption of primary antibodies or probes.

This kit is a 4-color fluorescent labeling kit, including HF 488, HF 546, HF 633, and DAPI, which can realize multiplex labeling of samples. After one single-color fluorescent labelling, use an antibody stripping method (such as microwave heating treatment in an appropriate buffer) to remove the non-covalently bound antibody, then in the next labelling, employ another primary antibody, fluorescein tyramide, and so on.

Our Tyramide Signal Amplification (TSA) product family also includes several other single-staining kits: K1050 with fluorescein for detection at excitation and emission wavelengths of 494 nm/517 nm, K1051 with Cyanine 3 for detection at excitation and emission wavelengths of 550 nm/570 nm, K1052 with Cyanine 5 at excitation and emission wavelengths of 648 nm/667 nm, and K4301 with HyperFluor 488, which can detect the signal at the excitation and emission wavelengths of 495 nm/519 nm. You can purchase TSA kits with different wavelengths according to your own requirements.