TUNEL Apoptosis Detection Kit (DAB)
The TUNEL Apoptosis Detection Kit (DAB) can be used to detect nuclear DNA fragmentation in tissues or cells during apoptosis. Intracellular DNA endonuclease enzymes are activated during apoptosis, severing genomic DNA between nucleosomes, and DNA is degraded into fragments of 180-200 bp or other integer multiples. The TUNEL kit uses TdT enzyme to incorporate biotin-dUTP onto the 3 ́-OH ends of fragmented DNA, then uses HRP-labeled streptavidin (Streptavidin-HRP) to bind the biotin-dUTP. Finally, add the DAB substrate to detect apoptosis.
This kit can directly use a common light microscope to detect, which is convenient to use. At the same time, this kit has a wide range of applications, which can be used to detect apoptosis in frozen or paraffin sections, as well as apoptosis in cultured adherent cells or suspension cells.
Figure 1: Detection results of untreated, Camptothecin-treated and Dnase I-treated (positive control) cells by this kit.
|
Components |
20 rxns |
50 rxns |
100 rxns |
Storage |
|
TdT Enzyme |
100 μL |
250 μL |
500 μL |
-20°C |
|
Bition-dUTP |
100 μL |
250 μL |
500 μL |
-20°C away from light |
|
5× Reaction buffer |
160 μL |
400 μL |
800 μL |
-20°C |
|
TdT Dilution Buffer (optional) |
500 μL |
1 mL |
2 x 1 mL |
-20°C |
|
Streptavidin-HRP |
22 μL |
55 μL |
110 μL |
-20°C |
|
Streptavidin-HRP Dilution Buffer |
1 mL |
2 x 1.25 mL |
4 x 1.25 mL |
-20°C away from light |
|
DAB-A Solution |
6 mL |
15 mL |
30 mL |
-20°C away from light |
|
DAB-B Solution |
6 mL |
15 mL |
30 mL |
-20°C away from light |
|
Protein K (2 mg/mL) |
40 μL |
100 μL |
200 μL |
-20°C |
|
Dnase I |
5 μL |
10 μL |
20 μL |
-20°C |
|
10× Dnase I Buffer |
100 μL |
250 μL |
500 μL |
-20°C |
|
Shipping: Dry ice Shelf life: 1 year |
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