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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
The TUNEL Apoptosis Detection Kit (DAB) can be used to detect nuclear DNA fragmentation in tissues or cells during apoptosis. Intracellular DNA endonuclease enzymes are activated during apoptosis, severing genomic DNA between nucleosomes, and DNA is degraded into fragments of 180-200 bp or other integer multiples. The TUNEL kit uses TdT enzyme to incorporate biotin-dUTP onto the 3 ́-OH ends of fragmented DNA, then uses HRP-labeled streptavidin (Streptavidin-HRP) to bind the biotin-dUTP. Finally, add the DAB substrate to detect apoptosis.
This kit can directly use a common light microscope to detect, which is convenient to use. At the same time, this kit has a wide range of applications, which can be used to detect apoptosis in frozen or paraffin sections, as well as apoptosis in cultured adherent cells or suspension cells.
Figure 1: Detection results of untreated, Camptothecin-treated and Dnase I-treated (positive control) cells by this kit.
Components
20 rxns
50 rxns
100 rxns
Storage
TdT Enzyme
100 μL
250 μL
500 μL
-20°C
Bition-dUTP
-20°C away from light
5× Reaction buffer
160 μL
400 μL
800 μL
TdT Dilution Buffer (optional)
1 mL
2 x 1 mL
Streptavidin-HRP
22 μL
55 μL
110 μL
Streptavidin-HRP Dilution Buffer
2 x 1.25 mL
4 x 1.25 mL
DAB-A Solution
6 mL
15 mL
30 mL
DAB-B Solution
Protein K (2 mg/mL)
40 μL
200 μL
Dnase I
5 μL
10 μL
20 μL
10× Dnase I Buffer
Shipping: Dry ice Shelf life: 1 year