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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
APExBIO's Tyramine Signal Amplification (TSA) system can be used to detect low-abundance targets in fluorescent immunocytochemistry (ICC), immunohistochemistry (IHC) and in situ hybridisation (ISH) techniques, increasing signal sensitivity by up to 100-fold. The TSA Fluorescence Kit uses horseradish peroxidase (HRP) to directly catalyse covalent deposition of fluorescent groups around the immobilised enzyme. The labelling process is rapid (less than 10 minutes) and the deposited labels can be viewed directly under a standard or confocal microscope.The TSA Fluorescence Kit can also be used in combination with antifluorescence for brightfield microscopy of enzyme concatenates and suitable colour-generating substrates.The use of the TSA reagent significantly increases the signal sensitivity compared to normal experiments, while maintaining a consistent level of specificity and resolution. In addition, TSA reagents can significantly reduce the consumption of primary antibodies or probes.
This product is a ready-to-use reagent for the preparation of tyramide fluorescence working solutions in TSA products (e.g., K1050, etc.).
Keep 1X Amplification Diluent at 4°C. Shelf life: 12 months upon receipt.
