Cell Counting Kit-8 (CCK-8)

The Cell Counting Kit-8 (CCK-8) uses a water-soluble tetrazole salt WST-8 that is bioreduced in live cells to produce a methane dye. This enzymatic reduction relies on intracellular dehydrogenase activity, allowing for a direct correlation between the amount of methane produced and the number of viable cells. The water solubility of the resulting methane simplifies the assay procedure and can be quantified by a microplate reader. This kit is commonly used in biomedical research to assess cell proliferation, viability, and cytotoxicity in vitro. Compared to methods like MTT, XTT, MTS, or WST-1, CCK-8 offers increased sensitivity and ease of use.

More Sensitive than MTT, MTS, or WST-1

No Toxicity to Cell

Simpler Steps, No organic solvents required

Stable One Bottle Solution, ready-to-use

Advantages