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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Our product 2X Hyperfusion plus master mix offers superior performance for major PCR applications with high fidelity, powerful amplification ability and faster extension speed. Hyperfusion plus DNA polymerase is composed of a DNA-binding domain fused to a Pyrococcus-like proofreading polymerase. Its unique structure, a novel Pyrococcus-like enzyme fused with a processivity-enhancing domain, increases fidelity and speed. The high fidelity makes hyperfusion plus DNA polymerase a superior choice for cloning or other subsequent applications. Hyperfusion plus DNA polymerase possesses one of the most accuracy with an error rate that is 50-fold lower compared to Taq DNA Polymerase and 6-fold lower than Pyrococcus Furiosus DNA Polymerase (Pfu).
Hyperfusion plus DNA polymerase possesses 5´→ 3´ polymerase activity and 3´→ 5´ exonuclease activity. It will generate blunt-ended products in the amplification products without an A overhang which appears in the product amplified with Taq polymerase. The polymerase has been capable of amplifying genomic fragment as long as 20.1 kb in our assays. In PCR reaction, elongation rate of hyperfusion plus DNA polymerase is about 15-30 sec/kb depending on the complexity of the amplicon.
2X Hyperfusion plus master mix is a ready-to-use 2×premix solution containing polymerase, dNTPs and an already optimized buffer system.
Store the components at -20°C for 12-24 months.
