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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Annexin V is a cellular protein, which plays important roles in the inhibition of the activity of phospholipase A1 and blood coagulation by competing for phosphatidylserine (PS) binding sites with prothrombin. Annexin V has a high affinity to PS and is used as a probe to detect cells that have expressed PS on the cell surface. After initiating apoptosis, cells translocate phosphatidylserine (PS) from the inner face of the plasma membrane to the cell surface soon, which can be easily detected by Annexin V.
Annexin V-Cy5/DAPI Apoptosis Kit uses a fluorescent conjugate of Annexin V that can easily detect PS on the cell surface after initiating apoptosis. The one-step staining procedure needs only 10-20 minutes. The result can be analyzed by fluorescence microscopy or by flow cytometry. The Annexin V-Cy5/PI Apoptosis Kit can differentiate apoptosis vs necrosis when performing both Annexin V-Cy5 and DAPI staining.
Figure 1: Cell apoptosis is detected by this kit
Store the kit at 2-8°C stable for 6 months. Annexin V-Cy5 and DAPI should be stored away from light. Do not freeze Annexin V-Cy5.
Fast and convenient.
Simple one step staining procedure in 10-20 minutes.
Kit can differentiate apoptosis vs necrosis when performing both Annexin V-Cy5 and DAPI staining.
