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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Cardiogreen, also known as indocyanine green, is a nontoxic tricarbocyanine dye, with a peak spectral absorption at 790 nm. Cardiogreen administered intravenously rapidly binds to its principle carrier, plasma protein, and then becomes confined to the vascular space. Cardiogreen has been commonly used in medical diagnostics for measuring cardiac output, liver blood flow, and hepatic function, as well as for ophthalmic angiography. In addition, cardiogreen can also be used as a photosensitizer in the photodynamic therapy (PDT) for treating diseases like periodontal infections.
References:
1. "Indocyanine green solution". NIH. National Cancer Institute. Retrieved 20 August 2021.
2. Gharesi S, Pourhajibagher M, Chiniforush N, et al. Effect of photodynamic therapy based on indocyanine green on expression of apoptosis-related genes in human gingival fibroblast cells. Photodiagnosis and Photodynamic Therapy , 2017, 19: 33-36.
Cell lines
Human gingival fibroblast (HGF) cells
Reaction Conditions
1000 μg/mL cardiogreen for 5 min incubation
Applications
Cardiogreen PDT with the exposure time of 60s for the diode laser would appear to be an inducer of apoptosis in HGF cells at transcriptome level.
Note
The technical data provided above is for reference only.