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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Cysteine-containing aspartate proteolytic enzymes (Caspase) are a family of cysteine proteases that play important roles in apoptosis, necrosis, and inflammation. Caspase-2 is considered to be an upstream initiator of apoptosis signaling, and activation of caspase-2 can trigger a series of downstream events, including activation of executor caspases (e.g., caspase-3, caspase-7), thereby promoting the execution of apoptosis.
Caspase-2 Fluorometric Assay Kit provides a convenient and simple way for detecting the VDVAD-dependent caspase activity. VDVAD-AFC (AFC:7-amino-4-trifluoromethyl coumarin) emits blue light (λmax = 400 nm); while cleavage of VDVAD-AFC by Caspase-2 or related caspases, free AFC emits a yellow-green fluorescence (λmax = 505 nm), which can be quantified by using a microplate reader or a fluorometer. Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control determines the fold increase in Caspase-2 activity.
K2016-25T
K2016-100T
K2016-200T
K2016-400T
Cell Lysis Buffer
25 mL
100 mL
2X Reaction Buffer
2 mL
4 X 2 mL
16 mL
32 mL
VDVAD-AFC (1 mM)
125 µL
500 µL
2 X 0.5 mL
2 X 1 mL
DTT (1 M)
100 µL
400 µL
Store the kit at -20°C, stable for 6 months. VDVAD-AFC (1 mM) should be stored away from light.