JavaScript seems to be disabled in your browser. For the best experience on our site, be sure to turn on Javascript in your browser.
Tel: +1-832-696-8203
Email: [email protected]
Worldwide Distributors
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Cysteine-containing aspartate proteolytic enzymes (Caspase) are a family of cysteine proteases that play important roles in apoptosis, necrosis, and inflammation. Caspase-9 is a priming type of caspase that initiates endogenous apoptosis, which is apoptosis of the mitochondrial pathway. When cells are stressed, Cytochrome c is released into the cytoplasm and binds to the apoptotic protein activator 1 (Apaf-1) to form a complex. Caspase-9 was then recruited into this complex, forming the apoptotic bodies. In apoptotic bodies, caspase-9 is activated by self-cleavage. Activated caspase-9 further activates downstream effector caspases, such as caspase-3 and caspase-7.
Caspase-9 Colorimetric Assay Kit provides a convenient and simple method for the detection of LEHD-dependent caspase activity. When Caspase-9 or related caspases cleave LEHD from the labeled substrate LEHD-p-nitroaniline (LEHD-pNA), the light emission of free pNA can be measured at 405 nm or 400 nm using a microplate reader or spectrophotometer. The absorbance of free pNA in apoptotic samples was compared to an uninduced control to determine a fold increase in Caspase-9 activity.
Figure 1: Reference standard curve for this kit
K2019-25T
K2019-50T
K2019-100T
Reagent I
20 mL
25 mL
Reagent II
30 mL
60 mL
120 mL
Reagent III
0.25 mL
0.55 mL
2 X 0.55 mL
pNA Standard (5 mM)
1 mL
Store the kit at -20°C, stable for 6 months. Reagent III and pNA Standard (5 mM) should be stored away from light.