Caspases (Cysteine-dependent aspartate-directed proteases) are a family of cysteine proteases that play important roles in apoptosis, inflammation and necrosis. Sequential activation of caspases plays a critical role in cell apoptosis. Caspase-1, 2, 3, 4, 5, 6, 7, 8, 9 and 10 are members of caspases family. Caspase-1, 4 and 5 are involved in T-cell maturation. Caspase-2, 8, 9 and 10 are initiator caspases that cleave inactive pro-forms of effector caspases into active caspases. Caspase-3, 6 and 7 are effector caspases that cleave other protein substrates within the cell to trigger the apoptotic process.
Caspase Fluorometric Substrate Set II Plus is composed of ready-to-use AFC-labeled substrates for caspase-1, 2, 3/7, 4, 5, 6, 8, 9 and 10 of caspase family proteases. The kit is used to detect activities of members of caspase family proteases. All substrates are provided in liquid form.
Store at -20°C.