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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Akt, also known as protein kinase B, is a serine/threonine-specific protein kinase that can be activated by insulin and various growth factors. Akt plays important roles in multiple cellular processes such as apoptosis, cell proliferation, glucose metabolism, transcription and cell migration. Akt is involved in PI3 kinase pathway.
The KinaseSTAR Akt Activity Assay Kit provides a specific and simple way for detection of Akt activity based on Western Blot method. The assay uses an Akt-specific antibody to immunoprecipitate Akt from tissue or cell lysates. The kinase reaction conducted to detect Akt activity is performed using recombinant GSK-3α as substrate. The phosphorylation of GSK-3α is analyzed by Western blot method using a phospho-GSK-3α specific antibody. The kit specifically detects Akt1, Akt2, and Akt3 activities, without other kinase activity can be detected.