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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
The CFDA SE (carboxyfluorescein diacetate succinimidyl ester) Cell Tracer Kit is usually used in fluorescence analyses as a long-term tracer of cells. The Kit can be used for in vitro and in vivo labeling of cells to determine whether or not a cell is proliferating, especially.
CFDA SE is a cell-permeable dye, which could bind covalently to all free amines on the surface and inside of cells. It shows little cytotoxicity, with minimal observed effect on the proliferative ability or biology of cells. The intense fluorescent staining provided by CFDA SE which distinguish labeled cells from auto-fluorescence can enable the visualization even after several days post stain, making it an ideal stain for cell tracing studies.
Approximate fluorescence excitation/emission maxima: 492/517 nm, after hydrolysis.
Superior performance—the kit allows for bright, uniform staining of cells Long-term retention—dye is well-retained in cells even after several days post stain Non-toxic—minimal cytotoxicity for in vivo and in vitro experiment Simple and convenient staining method