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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Cyanine 5-dUTP is a Cyanine 5-conjugated analog of deoxyuridine triphosphate (dUTP), used for direct enzymatic labeling of nucleic acid probes. Cyanine 5-dUTP can be incorporated into DNA probes by standard nick translation, random priming and polymerase chain reactions. DNA probes generated by these methods are suitable for identification of specific sequences by in situ hybridization procedures and direct fluorescence detection. A Cyanine 5-labeled probe can also be used in combination with probes labeled with other fluorochromes to obtain spectrally distinct signals. DNA probes labeled with Cyanine 5-dUTP and Cyanine 3-dUTP have been successfully used in fluorescence in situ hybridization experiments, making multisequence analysis a simple, powerful and practical tool in many areas of research and clinical diagnostics.
Reference:
1. Yu H, Chao J, Patek D, et al. Cyanine dye dUTP analogs for enzymatic labeling of DNA probes. Nucleic Acids Research, 1994, 22(15): 3226-3232.