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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
EDTA (also known as edetic acid or etheylenediaminetetraacetic acid) is a chelating agent that binds certain metal ions (e.g. lead, calcium, magnesium and iron). EDTA has a variety of applications in laboratory, including preventing blood samples from clotting, keeping bacteria from forming a biofilm (thin layer stuck to a surface), as well as eliminating contaminating divalent cations and inhibiting protease activity in the process of protein purification. In addition, EDTA has also been applied in chelation therapy, acting by binding certain heavy metals and minerals such as lead and calcium and removing them from the body.
References:
1. Definition of EDTA - NCI Dictionary of Cancer Terms. National Cancer Institute. Retrieved 28 August 2021.
2. Mónico A, Martínez-Senra E, Cañada FJ, et al. Drawbacks of Dialysis Procedures for Removal of EDTA. PLoS One, 2017, 12(1): e0169843.