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Worldwide Distributors
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
P16 gene is an essential gene in the cell cycle, which is directly involved in the regulatory signaling pathway of the cell cycle and plays an important role in the formation and development of tumors. It can inhibit innate or acquired carcinogenic mutations in cells, inhibit the growth and spread of tumor cells by obstructing cell cycle progression and inducing cell aging. The inactivated or missing p16 gene has been linked to the development of a variety of tumors, including melanoma, pancreatic cancer, and cervical cancer.
EZ Cap™ Human P16 mRNA is provided at a concentration of ~1mg/mL with Cap1 structure, expressing human P16 protein. There are currently two ways to cap mRNA: One is co-transcription method, by adding Cap analogues into the transcription process. The other is enzymatic Capping. After transcription, Cap0 capping is performed by Vaccinia virus Capping Enzyme (VCE), GTP and S-adenosylmethionine (SAM). The Cap0 is then generated into the Cap1 through 2´-O-Methyltransferase and SAM. Cap1 Capping can also be performed by adding VCE, 2´-O-Methyltransferase, GTP and SAM in a one-step process. Cap 1 structure is more ideal for mammalian systems and possess higher transcription efficiency than Cap 0 structure. The addition of poly (A) tail increases the stability and lifetime of the mRNA in vitro and in vivo. Poly (A) tail also plays an important role in enhancing the efficiency of translation initiation.
mRNA Length
726 nucleotides
Concentration
~1 mg/mL
Buffer
1 mM Sodium Citrate, pH 6.4
Storage
-40°C or below
General tips
Please dissolve it on ice and protect from RNase carefully. Avoid repeated freeze/thaw cycles as possible. Don’t vortex. Upon first use, centrifuge the tube softly and aliquot it into several single use portions. Use RNase-free reagents and materials with appropriate RNase-free technique. Don’t add to the media with serum unless mixing with a transfection reagent.
Shipping Condition
ship with dry ice
