JavaScript seems to be disabled in your browser. For the best experience on our site, be sure to turn on Javascript in your browser.
Tel: +1-832-696-8203
Email: [email protected]
Worldwide Distributors
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Folinic acid, clinically known as leucovorin, is a ubiquitous member of biological folate pools, usually accounting for 10 ~ 20% of total cellular folates. Folinic acid can reduce methotrexate (MTX)-induced cytogenetic damage, and appears to be an anticlastogenic agent. Folinic acid serves as a rescue agent used alone or in combination with 5-fluorouracil to decrease MTX-induced toxicity.
References:
1. Keshava C, Keshava N, Whong WZ, et al. Inhibition of methotrexate-induced chromosomal damage by folinic acid in V79 cells. Mutation Research, 1998, 397(2): 221-228.
2. Iqbal MP, Ahmed M, Umer M, et al. Effect of methotrexate and folinic acid on skeletal growth in mice. Acta Paediatrica, 2003, 92(12): 1438-1444.
Cell lines
V79 cells
Reaction Conditions
5 or 50 μg/ml folinic acid for 40 h incubation
Applications
MTX alone induced a concentration-related increase in the percentages of micronucleated binucleated cells (MNBN) and aberrant cells (Abs). The addition of folinic acid at 50 μg/ml significantly reduced MNBN by 40 ~ 68% and Abs by 36 ~ 77%. Inhibition was also seen at 5 μg/ml folinic acid (12 to 54% for MNBN and 20 to 61% for Abs).
Animal models
Balb/c young growing male mice, 11.9 ± 0.25 g
Dosage form
7.0 mg/kg
Injected intraperitoneally every second day for 3 weeks
Chronic administration of MTX induced suppression of skeletal growth in mice. Folinic acid treatment following MTX administration appeared to reverse this growth inhibition.
Note
The technical data provided above is for reference only.
