Glutathione S-transferase (GST) is a key enzyme in the glutathione binding reaction and catalyzes the initial step of the glutathione binding reaction. GST is often used to construct fusion proteins in the field of genetic engineering research, because the GST fusion protein expression system has the characteristics of efficient expression of the target protein, good solubility of the expression product, and easy separation and purification of the product.
GST-tagged recombinant proteins can be specifically bound to GST-tag Purification Resin by GST, while other proteins cannot be bound, and GST-tagged recombinant proteins can be eluted by excess reduced glutathione (GSH) solution, thereby achieving GST-tagged protein isolation and purification. Since the purification process is always maintained under mild and non-denaturing conditions, the obtained target protein can maintain its own biological activity, so it can be used for routine structural and functional studies, antibody production, and protein-protein interactions, protein-nucleic acid interactions, etc.
This product is a simple, rapid, efficient and highly specific kit for the purification of GST-tagged proteins, which provides the relevant reagents and affinity chromatography column empty column tubes required for the purification of GST-tagged proteins, which brings great convenience to the purification of GST-tagged proteins. Compared with most similar products currently on the market, non-specific protein binding is lower, pressure tolerance is stronger, and GSH conjugation is more stable.
This product can purify up to 10 GST-tagged recombinant proteins, with a maximum purification volume of approximately 12 mg per protein. For GST-tagged recombinant proteins with a molecular weight of 50 kD, a maximum of 10 proteins of approximately 4-6 mg can be purified in one package. Without the use of an affinity chromatography column empty column tube, this kit is sufficient for 500 small purifications of GST-tagged proteins with reference to these instructions.
