JavaScript seems to be disabled in your browser. For the best experience on our site, be sure to turn on Javascript in your browser.
Tel: +1-832-696-8203
Email: [email protected]
Worldwide Distributors
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Hydroxy benzotriazole, abbreviated HOBt, is anorganic compoundthat is a derivative of benzotriazole. HOBt, as a commercial product, is a white crystalline powder contains some water (~11.7% wt as the HOBt monohydrate crystal). It is mainly used to suppress the racemization of single-enantiomerchiral molecules and to improve the efficiency ofpeptide synthesis. Automated peptide synthesisinvolves the condensation of the amino group of protected amino acidswith the activated ester. HOBt can be used to produce activated esters such as N-hydroxysuccinimide ester. These esters are insoluble and react with amines at ambient temperature to give amides [1]. HOBt is also used for the synthesis of amides from carboxylic acidsaside from amino acids. These substrates may not be convertible to theacyl chlorides [2]. For instance amide derivatives of ionophoric antibiotics have been prepared in this way [3].
References:[1]. Knig W, Geiger R. EineneueMethodezurSynthese von Peptiden: Aktivierung der CarboxylgruppemitDicyclohexylcarbodiimidunterZusatz von 1‐Hydroxy‐benzotriazolen[J]. ChemischeBerichte, 1970, 103(3): 788-798.[2]. Myers A G, Yang B H, Chen H. Transformation of Pseudoephedrine Amides into Highly Enantiomerically Enriched Aldehydes, Alcohols, and Ketones[J]. Organic Syntheses, 2000: 29-29.[3]. owicki D, Huczyński A, Ratajczak-Sitarz M, et al. Structural and antimicrobial studies of a new N-phenylamide of monensin A complex with sodium chloride[J]. Journal of Molecular Structure, 2009, 923(1): 53-59.